Tatiana Kostrominova, Ph.D.
Associate Professor of Anatomy and Cell Biology
Course Director for Medical Histology and Cell Biology in Step 2 Human Structure
- Ph.D. in Cell Biology, Institute of Cytology Russian Academy of Sciences, St. Petersburg, Russia, 1991
- Postdoctoral Fellow, University of Connecticut, Farmington, 1992-1994
- Postdoctoral Research Associate, Washington University, Saint Louis, 1994-1998
- Postdoctoral Fellow, University of Michigan, Ann Arbor, 1998-2005
- Research Investigator, University of Michigan, Ann Arbor, 2005-2006
- The American Physiological Society, 2003 to present
- The American Association of Anatomists, 2008 to present
The main focus of my research is to study changes of gene and protein expression during denervation and aging and their effects on the decline of functional activity of muscle and tendon. One of the features of skeletal muscles of old animals is spontaneous denervation of some of the muscle fibers. The number of denervated fibers shows a progressive increase with advanced age. Increased denervation of fibers leads to muscle atrophy, weakness, and loss of physiological functions. Tendons also show significant changes with aging. My goal is to elucidate mechanisms of gene regulation that are critical for the prevention of skeletal muscle and tendon functional decline associated with aging and denervation.
I am also interested in studies on the effect of obesity and aging on skeletal muscles. I use rhesus monkeys, Zucker rats and Ossabaw pigs as experimental models for these studies. We showed previously that in rhesus monkeys, the obesity was associated with a decline in fast oxidative fibers in vastus lateralis muscles during aging. In Ossabaw pigs, obesity promoted the accumulation of the intramyocellular lipid droplets that could interfere with muscle functions. My most recent studies include the effect of obesity and environmental pollutants-obesogenes on the developmental changes in Zebrafish. In future studies, I will be evaluating age- and /or obesity-associated changes in structure and gene expression patterns in skeletal muscles, motor neurons, and neuromuscular junctions in these experimental models.
Selected Peer-reviewed publications
1. Kostrominova, T.Y., Reiner D.S., Haas R.H., Ingermanson R., and McDonough P.M. (2013) Automated Methods for the Analysis of Skeletal Muscle Fiber Size and Metabolic Type. Book Chapter. International Review of Cell and Molecular Biology, Chapter 7, 306.
2. Kostrominova* T.Y. and Brooks S. (2013) Age-related changes in structure and extracellular matrix protein expression levels in rat tendons. Age. Epab ahead of print.
3. Ma J., Smietana M.J., Kostrominova T.Y., Wojtys E.M., Larkin L.M. and Arruda E.M. (2012) Three-Dimensional Engineered Bone-Ligament-Bone Constructs for Anterior Cruciate Ligament Replacement. Tissue Engineering 18: 103-106.
4. Clark B.A., Alloosh M., Wenzel J.W., Sturek M. and Kostrominova* T.Y. (2011) Effect of diet-induced obesity and metabolic syndrome on skeletal muscles of Ossabaw miniature swine. Am J Physiol Endocrinol Metab. 300: E848-E857.
5. Kostrominova T.Y. (2011) Application of WGA lectin staining for visualization of the connective tissue in skeletal muscle, bone, and ligament/tendon studies. Microsc. Res. Tech. 74: 18-22.
6. Kostrominova* T.Y., Coleman S., Oleson F.B., Faulkner J.A. and Larkin L.M. (2010) Effect of daptomycin on primary rat muscle cell cultures in vitro. In Vitro Cell Dev. Biol. Anim. 46(7): 613-8.
7. Kostrominova T.Y. (2010) Advanced age-related denervation and fiber type grouping in skeletal muscle of SOD1 knockout mice. Free. Radic Biol. Med. 49: 1582-1593.
8. Kostrominova* T.Y., Hassett C.A., Rader E., Davis C., Larkin L.M., Coleman S., Oleson F.B. and Faulkner, J.A. (2010) Characterization of Skeletal Muscle Effects Associated with Daptomycin in Rats. Muscle Nerve. 42: 385-393
9. Kostrominova T.Y., Calve S., Arruda E.M., and Larkin L.M. (2009) Ultrastructure of Myotendinous Junctions in Tendon-Skeletal Muscle Constructs Engineered in vitro. Histol Histopathol. 24: 541-550.
10. Kostrominova, T.Y., Pasyk, K.A., Van Remmen, H., Richardson, A.G., and Faulkner, J.A. (2007) Adaptive changes in structure of skeletal muscles from adult Sod1 homozygous knockout mice. Cell Tissue Res 327: 595-605.
11. Kostrominova, T.Y., Dow, D.E., Dennis, R.G., Miller, R.A., and Faulkner, J.A. (2005) Comparison of Gene Expression of Two-Month Denervated, Two-Month Denervated-Stimulated and Control Rat Skeletal Muscles. Physiol. Genomics 22: 227-243.
12. Macpherson, P., Kostrominova, T.Y., Tang, H., and Goldman, D. (2002) Protein Kinase C and Calcium/Calmodulin-activated Protein Kinase II (CaMKII) Suppress Nicotinic Acetylcholine Receptor Gene Expression in Mammalian Muscle. A Specific Role for CaMKII in Activity-Dependent Gene Expression. J. Biol. Chem. 277: 15638-15646.
13. Kostrominova, T.Y., Macpherson,P.D., Carlson, B. M., and Goldman, D. (2000) Regulation of Myogenin Protein Expression in Denervated Muscles from Young and Old Rats. Am. J. Physiology 279: R179-R188.
14. Kostrominova, T.Y., and Tanzer, M.L. (1995) Temporal and Spatial Appearance of ?-Dystroglycan in Differentiated Mouse Myoblasts in Culture. J. of Cell. Biochem. 58: 527-534.